NECAB1 antibody (AA 16-296)

Catalog No. ABIN7600170

The Rabbit Polyclonal anti-NECAB1 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect NECAB1 in samples from Human, Mouse and Rat.

Quick Overview for NECAB1 antibody (AA 16-296) (ABIN7600170)

Target: NECAB1 (N-terminal EF-Hand Calcium Binding Protein 1 (NECAB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NECAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NECAB1 Antibody

Binding Specificity: AA 16-296

Purpose: Anti-NECAB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NECAB1 Antibody Picoband® (ABIN7600170). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NECAB1 recombinant protein (Position: E16-E296).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Wu, H. , Li, D. , Shan, Y. , Wan, B. , Hexige, S. , & Guo, J. , et al. (2007). Efcbp1/necab1, a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing ef-hand and antibiotic biosynthesis monooxygenase domains. Dna Seq, 18(1), 73-79. 2. Zhang, M. D. , Barde, S. , Szodorai, E. , Josephson, A. , Mitsios, N. , & Watanabe, M. , et al. (2016). Comparative anatomical distribution of neuronal calcium-binding protein (necab) 1 and -2 in rodent and human spinal cord. Brain Structure & Function, 221(7), 1-21. 3. Sugita, S. , Ho, A. , & T.C. Südhof. (2002). Necabs: a family of neuronal ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern. Neuroscience, 112(1), 51-63.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NECAB1

Target: NECAB1 (N-terminal EF-Hand Calcium Binding Protein 1 (NECAB1))

Alternative Name: NECAB1

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: NECAB1, also named as EFCBP1, is a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains Short Communication.

Molecular Weight: 41 kDa

Gene ID: 64168

UniProt: Q8N987