EIF3E antibody (AA 160-249)
Quick Overview for EIF3E antibody (AA 160-249) (ABIN7600190)
Target
See all EIF3E AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 160-249
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Purpose
- Anti-EIF3E Antibody Picoband®
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins.
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Characteristics
- Anti-EIF3E Antibody Picoband® (ABIN7600190). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human EIF3E recombinant protein (Position: A160-M249).
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 23477-80. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))
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Alternative Name
- EIF3E
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Background
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Synonyms: Eukaryotic translation initiation factor 3 subunit E ,eIF3e ,Eukaryotic translation initiation factor 3 subunit 6 ,Viral integration site protein INT-6 homolog,eIF-3 p48 ,EIF3E ,EIF3S6 , INT6 ,
Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .
Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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Molecular Weight
- 52 kDa
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Gene ID
- 3646
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UniProt
- P60228
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Pathways
- Ribonucleoprotein Complex Subunit Organization, Hepatitis C
Target
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