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EIF3E antibody (AA 160-249)

This Rabbit Polyclonal antibody specifically detects EIF3E in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN7600190

Quick Overview for EIF3E antibody (AA 160-249) (ABIN7600190)

Target

See all EIF3E Antibodies
EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity

  • 40
  • 23
  • 19
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
Human, Mouse

Host

  • 37
  • 2
  • 1
Rabbit

Clonality

  • 38
  • 2
Polyclonal

Conjugate

  • 28
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This EIF3E antibody is un-conjugated

Application

  • 32
  • 14
  • 7
  • 7
  • 6
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • Binding Specificity

    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 160-249

    Purpose

    Anti-EIF3E Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-EIF3E Antibody Picoband® (ABIN7600190). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human EIF3E recombinant protein (Position: A160-M249).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human, Mouse
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse
    ELISA, 0.1-0.5 μg/mL, -
    1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 23477-80. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

    Alternative Name

    EIF3E

    Background

    Synonyms: Eukaryotic translation initiation factor 3 subunit E ,eIF3e ,Eukaryotic translation initiation factor 3 subunit 6 ,Viral integration site protein INT-6 homolog,eIF-3 p48 ,EIF3E ,EIF3S6 , INT6 ,

    Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .

    Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

    Molecular Weight

    52 kDa

    Gene ID

    3646

    UniProt

    P60228

    Pathways

    Ribonucleoprotein Complex Subunit Organization, Hepatitis C
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