PCOLCE antibody (AA 162-449)

Catalog No. ABIN7600205

The Rabbit Polyclonal anti-PCOLCE antibody (ABIN7600205) specifically detects PCOLCE in WB, IHC, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for PCOLCE antibody (AA 162-449) (ABIN7600205)

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCOLCE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-PCOLCE Antibody

Binding Specificity: AA 162-449

Purpose: Anti-PCOLCE Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PCOLCE shares 75.6%,76.3% amino acid (aa) sequence identity with mouse,rat PCOLCE,respectively.

Characteristics: Anti-PCOLCE Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCOLCE recombinant protein (Position: R162-D449). Human PCOLCE shares 75.6% and 76.3% amino acid (aa) sequence identity with mouse and rat PCOLCE, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PCOLCE

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Alternative Name: PCOLCE

Background:

Background: Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

Gene Full Name: procollagen C-endopeptidase enhancer

Molecular Weight: 50 kDa

Gene ID: 5118

UniProt: Q15113