EEF2K antibody (AA 162-719)

Catalog No. ABIN7600209

This Rabbit Polyclonal antibody specifically detects EEF2K in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for EEF2K antibody (AA 162-719) (ABIN7600209)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-EEF2K Antibody

Binding Specificity: AA 162-719

Purpose: Anti-EEF2K Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EEF2K Antibody Picoband® (ABIN7600209). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EEF2K recombinant protein (Position: K162-A719).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arora, S., Yang, J.-M., Craft, J., Hait, W. Detection of anti-elongation factor 2 kinase (calmodulin-dependent protein kinase III) antibodies in patients with systemic lupus erythematosus. Biochem. Biophys. Res. Commun. 293: 1073-1076, 2002. 2. Autry, A. E., Adachi, M., Nosyreva, E., E. S., Los, M. F., Cheng, P., Kavalali, E. T., Monteggia, L. M. NMDA receptor blockade at rest triggers rapid behavioural antidepressant responses. Nature 475: 91-95, 2011. 3. Ryazanov, A. G., Ward, M. D., Mendola, C. E., Pavur, K. S., Dorovkov, M. V., Wiedmann, M., Erdjument-Bromage, H., Tempst, P., Parmer, T. G., Prostko, C. R., Germino, F. J., Hait, W. N. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Nat. Acad. Sci. 94: 4884-4889, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: EEF2K

Background:

Synonyms: Arachidonate 12-lipoxygenase, 12S-type,12S-LOX,12S-lipoxygenase,1.13.11.31,Lipoxin synthase 12-LO,3.3.2.-,Platelet-type lipoxygenase 12,ALOX12,12LO, LOG12,

Tissue Specificity: Expressed in vascular smooth muscle cells. .

Background: Eukaryotic elongation factor-2 kinase (eEF-2 kinase or eEF-2K), also known as calmodulin-dependent protein kinase III (CAMKIII) and calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is an enzyme that in humans is encoded by the EEF2K gene. This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Molecular Weight: 82 kDa

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling