GBA2 antibody (AA 17-869)

Catalog No. ABIN7600278

This Rabbit Polyclonal antibody specifically detects GBA2 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GBA2 antibody (AA 17-869) (ABIN7600278)

Target: GBA2 (Glucosidase, beta (Bile Acid) 2 (GBA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GBA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GBA2 Antibody

Binding Specificity: AA 17-869

Purpose: Anti-GBA2 Antibody Picoband®

Characteristics: Anti-GBA2 Antibody Picoband® (ABIN7600278). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GBA2 recombinant protein (Position: E17-Q869). Human GBA2 shares 88.5% and 90.2% amino acid (aa) sequence identity with mouse and rat GBA2, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Boot, R. G., Verhoek, M., Donker-Koopman, W., Strijland, A., van Marle, J., Overkleeft, H. S., Wennekes, T., Aerts, J. M. F. G. Identification of the non-lysosomal glucosylceramidase as beta-glucosidase 2. J. Biol. Chem. 282: 1305-1312, 2007. 2. Boukhris, A., Feki, I., Elleuch, N., Miladi, M. I., Boland-Auge, A., Truchetto, J., Mundwiller, E., Jezequel, N., Zelenika, D., Mhiri, C., Brice, A., Stevanin, G. A new locus (SPG46) maps to 9p21.2-q21.12 in a Tunisian family with a complicated autosomal recessive hereditary spastic paraplegia with mental impairment and thin corpus callosum. Neurogenetics 11: 441-448, 2010. 3. Hammer, M. B., Eleuch-Fayache, G., Schottlaender, L. V., Nehdi, H., Gibbs, J. R., Arepalli, S. K., Chong, S. B., Hernandez, D. G., Sailer, A., Liu, G., Mistry, P. K., Cai, H., Shrader, G., Sassi, C., Bouhlal, Y., Houlden, H., Hentati, F., Amouri, R., Singleton, A. B. Mutations in GBA2 cause autosomal-recessive cerebellar ataxia with spasticity. Am. J. Hum. Genet. 92: 245-251, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GBA2

Target: GBA2 (Glucosidase, beta (Bile Acid) 2 (GBA2))

Alternative Name: GBA2

Background: GBA2 is the gene that encodes the enzyme non-lysosomal glucosylceramidase in humans. This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism.

Molecular Weight: 105 kDa

Gene ID: 57704