DLL1 antibody (AA 18-239)

Catalog No. ABIN7600324

The Rabbit Polyclonal anti-DLL1 antibody has been validated for WB, ELISA, IHC, FACS, IF and ICC. It is suitable to detect DLL1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for DLL1 antibody (AA 18-239) (ABIN7600324)

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DLL1 Antibody

Binding Specificity: AA 18-239

Purpose: Anti-DLL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DLL1 Antibody Picoband® (ABIN7600324). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DLL1 recombinant protein (Position: Q18-K239).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chakrabarti, R., Celia-Terrassa, T., Kumar, S., Hang, X., Wei, Y., Choudhury, A., Hwang, J., Peng, J., Nixon, B., Grady, J. J., DeCoste, C., Gao, J., van Es, J. H., Li, M. O., Aifantis, I., Clevers, H., Kang, Y. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science 360: 1421 only, 2018. Note: Full Article Online. 2. Fischer-Zirnsak, B., Segebrecht, L., Schubach, M., Charles, P., Alderman, E., Brown, K., Cadieux-Dion, M., Cartwright, T., Chen, Y., Costin, C., Fehr, S., Fitzgerald, K. M., and 26 others. Haploinsufficiency of the Notch ligand DLL1 causes variable neurodevelopmental disorders. Am. J. Hum. Genet. 105: 631-639, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 4, MRC OX40, OX40 antigen, OX40L receptor, CD134, Tnfrsf4, Ox40, Txgp1l

Background: DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. This gene is mapped to chromosome 6q27.

Molecular Weight: 78 kDa

Gene ID: 28514

UniProt: O00548

Pathways: Notch Signaling, Stem Cell Maintenance