IFI16 antibody (AA 183-743)

Catalog No. ABIN7600373

This anti-IFI16 antibody is a Mouse Monoclonal antibody detecting IFI16 in WB, IF, ICC and FACS. Suitable for Human.

Quick Overview for IFI16 antibody (AA 183-743) (ABIN7600373)

Target: IFI16 (Interferon, gamma-Inducible Protein 16 (IFI16))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFI16 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2I3D7

Product Details anti-IFI16 Antibody

Binding Specificity: AA 183-743

Purpose: Anti-IFI16 Antibody Picoband® (monoclonal, 2I3D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IFI16 Antibody Picoband® (monoclonal, 2I3D7) (ABIN7600373). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IFI16 recombinant protein (Position: E183-K743).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Choubey, D., Snoddy, J., Chaturvedi, V., Toniato, E., Opdenakker, G., Thakur, A., Samanta, H., Engel, D. A., Lengyel, P. Interferons as gene activators: indications for repeated gene duplication during the evolution of a cluster of interferon-activatable genes on murine chromosome 1. J. Biol. Chem. 264: 17182-17189, 1989. 2. Kerur, N., Veettil, M. V., Sharma-Walia, N., Bottero, V., Sadagopan, S., Otageri, P., Chandran, B. IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi sarcoma-associated herpesvirus infection. Cell Host Microbe 9: 363-375, 2011. 3. Kingsmore, S. F., Snoddy, J., Choubey, D., Lengyel, P., Seldin, M. F. Physical mapping of a family of interferon-activated genes, serum amyloid P-component, and alpha-spectrin on mouse chromosome 1. Immunogenetics 30: 169-174, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IFI16

Target: IFI16 (Interferon, gamma-Inducible Protein 16 (IFI16))

Alternative Name: IFI16

Background:

Synonyms: Dihydrofolate reductase, DHFR

Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Background: Gamma-interferon-inducible protein Ifi-16 (Ifi-16) also known as interferon-inducible myeloid differentiation transcriptional activator is a protein that in humans is encoded by the IFI16 gene. This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 98 kDa

Gene ID: 3428

UniProt: Q16666

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Autophagy, Inflammasome