PIGS antibody (AA 184-504)

Catalog No. ABIN7600375

The Rabbit Polyclonal anti-PIGS antibody is suitable to detect PIGS in samples from Human and Rat. It has been validated for ELISA, WB, IF, FACS and ICC.

Quick Overview for PIGS antibody (AA 184-504) (ABIN7600375)

Target: PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGS antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PIGS Antibody

Binding Specificity: AA 184-504

Purpose: Anti-PIGS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PIGS Antibody (ABIN7600375). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIGS recombinant protein (Position: R184-D504).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PIGS

Target: PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))

Alternative Name: PIGS

Background:

Synonyms: Semaphorin-3E, SEMA3E, KIAA0331, SEMAH

Tissue Specificity: Detected in the sinusoidal epithelium in the red pulp of spleen. Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Background: GPI transamidase component PIG-S is an enzyme that in humans is encoded by the PIGS gene. This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

Molecular Weight: 65 kDa

Gene ID: 94005

UniProt: Q96S52

Pathways: Inositol Metabolic Process, SARS-CoV-2 Protein Interactome