SMPD3 antibody (AA 184-553)

Catalog No. ABIN7600377

This Rabbit Polyclonal antibody specifically detects SMPD3 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SMPD3 antibody (AA 184-553) (ABIN7600377)

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMPD3 Antibody

Binding Specificity: AA 184-553

Purpose: Anti-SMPD3 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-SMPD3 Antibody Picoband® (ABIN7599029). Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMPD3 recombinant protein (Position: F184-D553). Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse and rat SMPD3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aubin, I., Adams, C. P., Opsahl, S., Septier, D., Bishop, C. E., Auge, N., Salvayre, R., Negre-Salvayre, A., Goldberg, M., Guenet, J.-L., Poirier, C. A deletion in the gene encoding sphingomyelin phosphodiesterase 3 (Smpd3) results in osteogenesis and dentinogenesis imperfecta in the mouse. Nature Genet. 37: 803-805, 2005. 2. Guenet, J. L., Stanescu, R., Maroteaux, P., Stanescu, V. Fragilitas ossium: a new autosomal recessive mutation in the mouse. J. Hered. 72: 440-441, 1981. 3. Hofmann, K., Tomiuk, S., Wolff, G., Stoffel, W. Cloning and characterization of the mammalian brain-specific, Mg(2+)-dependent neutral sphingomyelinase. Proc. Nat. Acad. Sci. 97: 5895-5900, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Alternative Name: SMPD3

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Sphingomyelin phosphodiesterase 3 is an enzyme that in humans is encoded by the SMPD3 gene. SMPD3 (Sphingomyelin phosphodiesterase 3, also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91 % aa sequence identity with mouse SMPD3.

Molecular Weight: 75 kDa

Gene ID: 55512

Pathways: Hormone Transport