SMPD3 antibody (Middle Region)

Catalog No. ABIN2783713

There is 1 publication for this product available. The Rabbit Polyclonal anti-SMPD3 antibody is suitable to detect SMPD3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for SMPD3 antibody (Middle Region) (ABIN2783713)

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMPD3 Antibody

Binding Specificity: Middle Region

Purpose: SMPD3 Antibody - middle region

Sequence: RPPEADDPVP GGQARNGAGG GPRGQTPNHN QQDGDSGSLG SPSASRESLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SMPD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMPD3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49331-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SMPD3 antibody (ABIN2783713)

Tani, Hannun: "Analysis of membrane topology of neutral sphingomyelinase 2." in: FEBS letters, Vol. 581, Issue 7, pp. 1323-8, (2007) (PubMed).

Target Details for SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Alternative Name: SMPD3

Background:

Background Information: SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. SMPD3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.

Gene Name: Sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)

Alternative Symbols: NSMASE2

Protein Name: Sphingomyelin phosphodiesterase 3

Molecular Weight: 71kDa

Gene ID: 55512

NCBI Accession: NP_061137

UniProt: Q9NY59

Pathways: Hormone Transport