ALOX12 antibody (AA 186-628)

Catalog No. ABIN7600385

The Rabbit Polyclonal anti-ALOX12 antibody (ABIN7600385) specifically detects ALOX12 in WB, ELISA, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ALOX12 antibody (AA 186-628) (ABIN7600385)

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ALOX12 Antibody

Binding Specificity: AA 186-628

Purpose: Anti-ALOX12 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human ALOX12 shares 87.3%,86% amino acid (aa) sequence identity with mouse,rat ALOX12,respectively.

Characteristics: Anti-ALOX12 Antibody. Tested in ELISA, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALOX12 recombinant protein (Position: A186-R628). Human ALOX12 shares 87.3% and 86% amino acid (aa) sequence identity with mouse and rat ALOX12, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ALOX12

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Alternative Name: ALOX12

Background:

Background: ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNA Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

Gene Full Name: arachidonate 12-lipoxygenase, 12S type

Molecular Weight: 75 kDa

Gene ID: 239

UniProt: P18054

Pathways: Positive Regulation of Endopeptidase Activity