Muscarinic Acetylcholine Receptor M2 antibody (AA 381-466)

Catalog No. ABIN726695

The Rabbit Polyclonal anti-Muscarinic Acetylcholine Receptor M2 antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect Muscarinic Acetylcholine Receptor M2 in samples from Human and Mouse.

Quick Overview for Muscarinic Acetylcholine Receptor M2 antibody (AA 381-466) (ABIN726695)

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Muscarinic Acetylcholine Receptor M2 Antibody

Binding Specificity: AA 381-466

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ChRM2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Muscarinic Acetylcholine Receptor M2

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Alternative Name: ChRM2

Background:

Synonyms: HM2, Muscarinic acetylcholine receptor M2, CHRM2

Background: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3), this then triggers calcium ion release into the cytosol.

Gene ID: 1129

UniProt: P08172