GBP1 antibody (AA 190-592)

Catalog No. ABIN7600430

This anti-GBP1 antibody is a Rabbit Polyclonal antibody detecting GBP1 in WB, ELISA, IHC, IF, IP, ICC and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for GBP1 antibody (AA 190-592) (ABIN7600430)

Target: GBP1 (Guanylate Binding Protein 1, Interferon-Inducible (GBP1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GBP1 Antibody

Binding Specificity: AA 190-592

Purpose: Anti-GBP1 Antibody Picoband®

Characteristics: Anti-GBP1 Antibody Picoband® (ABIN7600430). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GBP1 recombinant protein (Position: E190-S592).

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cheng, Y.-S. E., Patterson, C. E., Staeheli, P. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP. Molec. Cell. Biol. 11: 4717-4725, 1991. 2. Gaudet, R. G., Zhu, S., Halder, A., Kim, B.-H., Bradfield, C. J., Huang, S., Xu, D., Maminska, A., Nguyen, T. N., Lazarou, M., Karatekin, E., Gupta, K., MacMicking, J. D. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 373: eabf8113, 2021. 3. Ghosh, A., Praefcke, G. J. K., Renault, L., Wittinghofer, A., Herrmann, C. How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP. Nature 440: 101-104, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GBP1

Target: GBP1 (Guanylate Binding Protein 1, Interferon-Inducible (GBP1))

Alternative Name: GBP1

Background: Interferon-induced guanylate-binding protein 1 is a protein that in humans is encoded by the GBP1 gene. It belongs to the dynamin superfamily of large GTPases. Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.

Molecular Weight: 68 kDa

Gene ID: 2633

UniProt: P32455

Pathways: Cellular Response to Molecule of Bacterial Origin