IFT88 antibody (AA 198-814)

Catalog No. ABIN7600458

This Rabbit Polyclonal antibody specifically detects IFT88 in WB, IHC, IF, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IFT88 antibody (AA 198-814) (ABIN7600458)

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT88 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-IFT88 Antibody

Binding Specificity: AA 198-814

Purpose: Anti-IFT88 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IFT88 Antibody Picoband® (ABIN7600458). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IFT88 recombinant protein (Position: E198-K814).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cano, D. A., Murcia, N. S., Pazour, G. J., Hebrok, M. orpk mouse model of polycystic kidney disease reveals essential role of primary cilia in pancreatic tissue organization. Development 131: 3457-3467, 2004. 2. Delaval, B., Bright, A., Lawson, N. D., Doxsey, S. The cilia protein IFT88 is required for spindle orientation in mitosis. Nature Cell Biol. 13: 461-468, 2011. 3. Huangfu, D., Liu, A., Rakeman, A. S., Murcia, N. S., Niswander, L., Anderson, K. V. Hedgehog signalling in the mouse requires intraflagellar transport proteins. Nature 426: 83-87, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IFT88

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Alternative Name: IFT88

Background:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: Intraflagellar transport protein 88 homolog is a protein that in humans is encoded by the IFT88 gene. It is mapped to 13q12.11. This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 94 kDa

Gene ID: 8100

UniProt: Q13099

Pathways: Hedgehog Signaling, Sensory Perception of Sound