SKP1 antibody (AA 2-128)

Catalog No. ABIN7600465

The Rabbit Polyclonal anti-SKP1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SKP1 in samples from Human, Mouse and Rat.

Quick Overview for SKP1 antibody (AA 2-128) (ABIN7600465)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SKP1 Antibody

Binding Specificity: AA 2-128

Purpose: Anti-SKP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SKP1 Antibody Picoband® (ABIN7600465). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SKP1 recombinant protein (Position: P2-K128).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arai, T., Kasper, J. S., Skaar, J. R., Ali, S. H., Takahashi, C., DeCaprio, J. A. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Nat. Acad. Sci. 100: 9855-9860, 2003. 2. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J. W., Elledge, S. J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274, 1996. 3. Chen, H., Thalmann, I., Adams, J. C., Avraham, K. B., Copeland, N. G., Jenkins, N. A., Beier, D. R., Corey, D. P., Thalmann, R., Duyk, G. M. cDNA cloning, tissue distribution and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. Genomics 27: 389-398, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1

Background:

Synonyms: S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, P19a, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, SKP1, EMC19, OCP2, SKP1A, TCEB1L

Tissue Specificity: Ubiquitously expressed.

Background: Skp1 (S-phase kinase-associated protein 1) is a small protein of approximately 160 amino acids. It is mapped to 5q31.1. This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds ly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein (s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Molecular Weight: 19 kDa

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases