TARBP2 antibody (AA 20-366)

Catalog No. ABIN7600548

This Rabbit Polyclonal antibody specifically detects TARBP2 in WB, IHC, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for TARBP2 antibody (AA 20-366) (ABIN7600548)

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TARBP2 Antibody

Binding Specificity: AA 20-366

Purpose: Anti-TRBP/TARBP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRBP/TARBP2 Antibody Picoband® (ABIN7600548). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRBP/TARBP2 recombinant protein (Position: E20-K366).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chendrimada, T. P., Gregory, R. I., Kumaraswamy, E., Norman, J., Cooch, N., Nishikura, K., Shiekhattar, R. TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. (Letter) Nature 436: 740-744, 2005. 2. Garre, P., Perez-Segura, P., Diaz-Rubio, E., Caldes, T., de la Hoya, M. Reassessing the TARBP2 mutation rate in hereditary nonpolyposis colorectal cancer. (Letter) Nature Genet. 42: 817-818, 2010. 3. Gatignol, A., Buckler-White, A., Berkhout, B., Jeang, K. T. Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR. Science 251: 1597-1600, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TARBP2

Target: TARBP2 (TAR (HIV-1) RNA Binding Protein 2 (TARBP2))

Alternative Name: TARBP2

Background:

Synonyms: Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, CCKBR, CCKRB

Tissue Specificity: Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.

Background: RISC-loading complex subunit TARBP2 is a protein that in humans is encoded by the TARBP2 gene. HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Molecular Weight: 44 kDa

Gene ID: 6895

UniProt: Q15633

Pathways: Regulatory RNA Pathways, Ribonucleoprotein Complex Subunit Organization