NFE2 antibody (AA 20-373)

Catalog No. ABIN7600550

This anti-NFE2 antibody is a Rabbit Polyclonal antibody detecting NFE2 in WB and ELISA. Suitable for Human.

Quick Overview for NFE2 antibody (AA 20-373) (ABIN7600550)

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NFE2 Antibody

Binding Specificity: AA 20-373

Purpose: Anti-NFE2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NFE2 Antibody Picoband® (ABIN7600550). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NFE2 recombinant protein (Position: E20-D373).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bannerman, R. M., Edwards, J. A., Kreimer-Birnbaum, M., McFarland, E., Russell, E. S. Hereditary microcytic anaemia in the mouse, studies in iron distribution and metabolism. Brit. J. Haemat. 23: 235-245, 1972. 2. Chan, J. Y., Cheung, M.-C., Moi, P., Chan, K., Kan, Y. W. Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization. Hum. Genet. 95: 265-269, 1995. 3. Chan, J. Y., Han, X.-L., Kan, Y. W. Isolation of cDNA encoding the human NF-E2 protein. Proc. Nat. Acad. Sci. 90: 11366-11370, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: NFE2

Background:

Synonyms: NAD-dependent protein lipoamidase sirtuin-4, mitochondrial, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein deacetylase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4, SIR2L4

Tissue Specificity: Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.

Background: Transcription factor NF-E2 45 kDa subunit is a protein that in humans is encoded by the NFE2 gene. Enables several functions, including WW domain binding activity, identical protein binding activity, and protein N-terminus binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development, negative regulation of bone mineralization, and negative regulation of syncytium formation by plasma membrane fusion. Part of protein-DNA complex.

Molecular Weight: 41 kDa

Gene ID: 4778

UniProt: Q16621