SETDB2 antibody (AA 20-714)

Catalog No. ABIN7600560

This Rabbit Polyclonal antibody specifically detects SETDB2 in WB, ELISA and FACS. It exhibits reactivity toward Rat and Mouse.

Quick Overview for SETDB2 antibody (AA 20-714) (ABIN7600560)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SETDB2 Antibody

Binding Specificity: AA 20-714

Purpose: Anti-CLLD8/Setdb2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CLLD8/Setdb2 Antibody Picoband® (ABIN7600560). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat CLLD8/Setdb2 recombinant protein (Position: K20-I714).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, Rat
1. Mabuchi, H., Fujii, H., Calin, G., Alder, H., Negrini, M., Rassenti, L., Kipps, T. J., Bullrich, F., Croce, C. M. Cloning and characterization of CLLD6, CLLD7, CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia. Cancer Res. 61: 2870-2877, 2001. 2. Zhang, Y., Leaves, N. I., Anderson, G. G., Ponting, C. P., Broxholme, J., Holt, R., Edser, P., Bhattacharyya, S., Dunham, A., Adcock, I. M., Pulleyn, L., Barnes, P. J., and 11 others. Positional cloning of a quantitative trait locus on chromosome 13q14 that influences immunoglobulin E levels and asthma. Nature Genet. 34: 181-186, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: Setdb2

Background:

Synonyms: Histone-lysine N-methyltransferase SETDB2, Chronic lymphocytic leukemia deletion region gene 8 protein, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SETDB2, C13orf4, CLLD8, KMT1F

Tissue Specificity: Highly expressed in reproductive organs, such as testis, ovary and prostate.

Background: SET domain bifurcated histone lysine methyltransferase 2 is a protein in humans encoded by the SETDB2 gene. It is mapped to 13q14.2. This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants.

Molecular Weight: 100 kDa

Gene ID: 100361710