MCM5 antibody (AA 206-723)

Catalog No. ABIN7600582

The Rabbit Polyclonal anti-MCM5 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect MCM5 in samples from Human, Mouse and Rat.

Quick Overview for MCM5 antibody (AA 206-723) (ABIN7600582)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MCM5 Antibody

Binding Specificity: AA 206-723

Purpose: Anti-MCM5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MCM5 Antibody Picoband® (ABIN7600582). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCM5 recombinant protein (Position: K206-H723).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, Human
1. Seroussi, E., Kedra, D., Kost-Alimova, M., Sandberg-Nordqvist, A.-C., Fransson, I., Jacobs, J. F. M., Fu, Y., Pan, H.-Q., Roe, B. A., Imreh, S., Dumanski, J. P. TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. Genomics 57: 380-388, 1999. 2. Tsuruga, H., Yabuta, N., Hashizume, K., Ikeda, M., Endo, Y., Nojima, H. Expression, nuclear localization and interactions of human MCM/P1 proteins. Biochem. Biophys. Res. Commun. 236: 118-125, 1997. 3. Vetro, A., Savasta, S., Russo Raucci, A., Cerqua, C., Sartori, G., Limongelli, I., Forlino, A., Maruelli, S., Perucca, P., Vergani, D., Mazzini, G., Mattevi, A., Stivala, L. A., Salviati, L., Zuffardi, O. MCM5: a new actor in the link between DNA replication and Meier-Gorlin syndrome. Europ. J. Hum. Genet. 25: 646-650, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background:

Synonyms: DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5, CDC46

Tissue Specificity: Ubiquitous.

Background: DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. It is mapped to 22q12.3. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Molecular Weight: 100 kDa

Gene ID: 4174

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA