PPP1R15B antibody (AA 211-657)

Catalog No. ABIN7600639

The Rabbit Polyclonal anti-PPP1R15B antibody has been validated for WB, ELISA and FACS. It is suitable to detect PPP1R15B in samples from Human.

Quick Overview for PPP1R15B antibody (AA 211-657) (ABIN7600639)

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R15B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PPP1R15B Antibody

Binding Specificity: AA 211-657

Purpose: Anti-PPP1R15B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PPP1R15B Antibody Picoband® (ABIN7600639). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPP1R15B recombinant protein (Position: Q211-D657).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Harding, H. P., Zhang, Y., Scheuner, D., Chen, J.-J., Kaufman, R. J., Ron, D. Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2-alpha) dephosphorylation in mammalian development. Proc. Nat. Acad. Sci. 106: 1832-1837, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPP1R15B

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Alternative Name: PPP1R15B

Background:

Synonyms: Protein NDRG3,N-myc downstream-regulated gene 3 protein,NDRG3,

Tissue Specificity: Ubiquitous. Highly expressed in brain. .

Background: PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.

Molecular Weight: 110 kDa

Gene ID: 84919

Pathways: ER-Nucleus Signaling