Cytokeratin 1 antibody (AA 215-431)

Catalog No. ABIN7600651

The Rabbit Polyclonal anti-Cytokeratin 1 antibody has been validated for WB, IHC, FACS, ELISA and IF. It is suitable to detect Cytokeratin 1 in samples from Human.

Quick Overview for Cytokeratin 1 antibody (AA 215-431) (ABIN7600651)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: AA 215-431

Purpose: Anti-Cytokeratin 1/KRT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cytokeratin 1/KRT1 Antibody Picoband® (ABIN7600651). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytokeratin 1/KRT1 recombinant protein (Position: L215-Q431).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chipev, C. C., Korge, B. P., Markova, N., Bale, S. J., DiGiovanna, J. J., Compton, J. G., Steinert, P. M. A leucine-to-proline mutation in the H1 subdomain of keratin 1 causes epidermolytic hyperkeratosis. Cell 70: 821-828, 1992. 2. Compton, J. G. Epidermal disease: faulty keratin filaments take their toll. Nature Genet. 6: 6-7, 1994. 3. Compton, J. G., DiGiovanna, J. J., Santucci, S. K., Kearns, K. S., Amos, C. I., Abangan, D. L., Korge, B. P., McBride, O. W., Steinert, P. M., Bale, S. J. Linkage of epidermolytic hyperkeratosis to the type II keratin gene cluster on chromosome 12q. Nature Genet. 1: 301-305, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: KRT1

Background:

Synonyms: Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1

Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Background: Keratin 1 is a Type II intermediate filament (IFs) of the intracytoplasmatic cytoskeleton. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molecular Weight: 66 kDa

Gene ID: 3848

UniProt: P04264