CD3 epsilon antibody (AA 22-113)

Catalog No. ABIN7600665

This Rabbit Polyclonal antibody specifically detects CD3 epsilon in FACS, WB, IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for CD3 epsilon antibody (AA 22-113) (ABIN7600665)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 22-113

Purpose: Anti-CD3 epsilon/Cd3e Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD3 epsilon/Cd3e Antibody. Tested in ELISA, Flow Cytometry , IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: Cd3e

Background:

Background: CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Gene Full Name: CD3 antigen, epsilon polypeptide

Molecular Weight: 23 kDa

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway