MAPK7 antibody (AA 22-816)

Catalog No. ABIN7600701

This Rabbit Polyclonal antibody specifically detects MAPK7 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for MAPK7 antibody (AA 22-816) (ABIN7600701)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MAPK7 Antibody

Binding Specificity: AA 22-816

Purpose: Anti-ERK5/MAPK7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ERK5/MAPK7 Antibody Picoband® (ABIN7600701). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ERK5/MAPK7 recombinant protein (Position: K22-P816).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hayashi, M., Kim, S.-W., Imanaka-Yoshida, K., Yoshida, T., Abel, E. D., Eliceiri, B., Yang, Y., Ulevitch, R. J., Lee, J.-D. Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure. J. Clin. Invest. 113: 1138-1148, 2004. 2. Lee, J.-D., Ulevitch, R. J., Han, J. Primary structure of BMK1: a new mammalian MAP kinase. Biochem. Biophys. Res. Commun. 213: 715-724, 1995. 3. Purandare, S. M., Lee, J.-D., Patel, P. I. Assignment of big MAP kinase (PRKM7) to human chromosome 17 band p11.2 with somatic cell hybrids. Cytogenet. Cell Genet. 83: 258-259, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Alternative Name: MAPK7

Background:

Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3

Tissue Specificity: Widely expressed.

Background: Mitogen-activated protein kinase 7 also known as MAP kinase 7 is an enzyme that in humans is encoded by the MAPK7 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

Molecular Weight: 115-120 kDa

Gene ID: 5598

UniProt: Q13164

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling