Cytokeratin 1 antibody (AA 223-439)

Catalog No. ABIN7600713

This Rabbit Polyclonal antibody specifically detects Cytokeratin 1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Mouse.

Quick Overview for Cytokeratin 1 antibody (AA 223-439) (ABIN7600713)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: AA 223-439

Purpose: Anti-Cytokeratin 1/Krt1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cytokeratin 1/Krt1 Antibody Picoband® (ABIN7600713). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse
Immunofluorescence, 5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: KRT1 keratin 1 (epidermolytic hyperkeratosis)". 2. Whittock NV, Ashton GH, Griffiths WA et al. (2001). "New mutations in keratin 1 that cause bullous congenital ichthyosiform erythroderma and keratin 2e that cause ichthyosis bullosa of Siemens". Br. J. Dermatol. 145(2): 330-5.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Krt1

Background:

Synonyms: Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1

Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Background: Keratin 1 (KRT1), also known as Cytokeratin 1, or Keratin, type II cytoskeletal 1, is a member of the keratin family. It is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants ofbullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. And this type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molecular Weight: 66 kDa

Gene ID: 16678

UniProt: P04104