Retinoid X Receptor alpha antibody (AA 226-462)

Catalog No. ABIN7600721

The Mouse Monoclonal anti-Retinoid X Receptor alpha antibody (Clone 5E7) (ABIN7600721) specifically detects Retinoid X Receptor alpha in WB and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Retinoid X Receptor alpha antibody (AA 226-462) (ABIN7600721)

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoid X Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 5E7

Product Details anti-Retinoid X Receptor alpha Antibody

Binding Specificity: AA 226-462

Purpose: Anti-Retinoid X Receptor alpha/RXRA Antibody (monoclonal, 5E7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Retinoid X Receptor alpha/RXRA Antibody (monoclonal, 5E7) (ABIN7600721). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RXRA recombinant protein (Position: A226-T462).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Retinoid X Receptor alpha

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Alternative Name: RXRA

Background:

Synonyms: Retinoic acid receptor RXR-alpha, Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha, RXRA, NR2B1

Tissue Specificity: Expressed in lung fibroblasts (at protein level). Expressed in monocytes. Highly expressed in liver, also found in kidney and brain.

Background: Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 55 kDa

Gene ID: 6256

UniProt: P19793

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C