PMPCB antibody (AA 23-479)

Catalog No. ABIN7600757

This anti-PMPCB antibody is a Mouse Monoclonal antibody detecting PMPCB in WB, IHC, FACS, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for PMPCB antibody (AA 23-479) (ABIN7600757)

Target: PMPCB (Peptidase (Mitochondrial Processing) beta (PMPCB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PMPCB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 9F13E4

Product Details anti-PMPCB Antibody

Binding Specificity: AA 23-479

Purpose: Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) (ABIN7600757). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. (Letter) Nature 340: 245-246, 1989. 2. Gordon, D. M., Shi, Q., Dancis, A., Pain, D. Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase. Hum. Molec. Genet. 8: 2255-2262, 1999. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/3/2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PMPCB

Target: PMPCB (Peptidase (Mitochondrial Processing) beta (PMPCB))

Alternative Name: PMPCB

Background:

Synonyms: ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

Molecular Weight: 43 kDa

Gene ID: 9512

UniProt: O75439

Pathways: SARS-CoV-2 Protein Interactome