NUPL1 antibody (AA 235-438)

Catalog No. ABIN7600785

This Rabbit Polyclonal antibody specifically detects NUPL1 in WB, ELISA and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for NUPL1 antibody (AA 235-438) (ABIN7600785)

Target: NUPL1 (Nucleoporin Like 1 (NUPL1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUPL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NUPL1 Antibody

Binding Specificity: AA 235-438

Purpose: Anti-NUP58 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUP58 Antibody Picoband® (ABIN7600785). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUP58 recombinant protein (Position: R235-E438).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chug, H., Trakhanov, S., Hulsmann, B. B., Pleiner, T., Gorlich, D. Crystal structure of the metazoan Nup62-Nup58-Nup54 nucleoporin complex. Science 350: 106-110, 2015. 2. Ishikawa, K., Nagase, T., Nakajima, D., Seki, N., Ohira, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 4: 307-313, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NUPL1

Target: NUPL1 (Nucleoporin Like 1 (NUPL1))

Alternative Name: NUP58

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: This gene encodes a member of the nucleoporin family that shares 87 % sequence identity with rat nucleoporin p58. The protein is localized to the nuclear rim and is a component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 69 kDa

Gene ID: 9818

Pathways: SARS-CoV-2 Protein Interactome