CYB5R3 antibody (AA 24-301)

Catalog No. ABIN7600822

The Rabbit Polyclonal anti-CYB5R3 antibody has been validated for WB, ELISA, IF, FACS and ICC. It is suitable to detect CYB5R3 in samples from Human, Mouse and Rat.

Quick Overview for CYB5R3 antibody (AA 24-301) (ABIN7600822)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-CYB5R3 Antibody

Binding Specificity: AA 24-301

Purpose: Anti-CYB5R3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CYB5R3 Antibody Picoband® (ABIN7600822). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CYB5R3 recombinant protein (Position: M24-F301).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aalfs, C. M., Salieb-Beugelaar, G. B., Wanders, R. J. A., Mannens, M. M. A. M., Wijburg, F. A. A case of methemoglobinemia type II due to NADH-cytochrome b5 reductase deficiency: determination of the molecular basis. Hum. Mutat. 16: 18-22, 2000. 2. Board, P. G., Pidcock, M. E. Methaemoglobinaemia resulting from heterozygosity for two NADH-methaemoglobin reductase variants: characterization as NADH-ferricyanide reductase. Brit. J. Haemat. 47: 361-370, 1981. 3. Bull, P. C., Shephard, E. A., Povey, S., Santisteban, I., Phillips, I. R. Cloning and chromosomal mapping of human cytochrome b(5) reductase (DIA1). Ann. Hum. Genet. 52: 263-268, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3

Background:

Synonyms: Protein Wnt-10a, WNT10A

Tissue Specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

Background: NADH-cytochrome b5 reductase 3 is an enzyme that in humans is encoded by the CYB5R3 gene. This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.

Molecular Weight: 34 kDa

Gene ID: 1727

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome