NRF1 antibody (AA 246-503)

Catalog No. ABIN7600874

This anti-NRF1 antibody is a Mouse Monoclonal antibody detecting NRF1 in WB, IHC, IF, ICC and FACS. Suitable for Human.

Quick Overview for NRF1 antibody (AA 246-503) (ABIN7600874)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2G4

Product Details anti-NRF1 Antibody

Binding Specificity: AA 246-503

Purpose: Anti-NRF1 Antibody Picoband® (monoclonal, 2G4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NRF1 Antibody Picoband® (monoclonal, 2G4) (ABIN7600874). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human NRF1 recombinant protein (Position: D246-Q503).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Efiok, B. J. S., Chiorini, J. A., Safer, B. A key transcription factor for eukaryotic initiation factor-2-alpha is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development. J. Biol. Chem. 269: 18921-18930, 1994. 2. Gopalakrishnan, L., Scarpulla, R. C. Structure, expression, and chromosomal assignment of the human gene encoding nuclear respiratory factor 1. J. Biol. Chem. 270: 18019-18025, 1995. 3. Virbasius, J. V., Scarpulla, R. C. Activation of the human mitochondrial transcription factor A gene by nuclear respiratory factors: a potential regulatory link between nuclear and mitochondrial gene expression in organelle biogenesis. Proc. Nat. Acad. Sci. 91: 1309-1313, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background:

Synonyms: Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

Tissue Specificity: Ubiquitously expressed with strongest expression in skeletal muscle.

Background: Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Molecular Weight: 54-70 kDa

Gene ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha