Liver Arginase antibody (AA 25-183)

Catalog No. ABIN7600888

The Mouse Monoclonal anti-Liver Arginase antibody has been validated for WB and FACS. It is suitable to detect Liver Arginase in samples from Human, Rat, Mouse and Monkey.

Quick Overview for Liver Arginase antibody (AA 25-183) (ABIN7600888)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 2B12

Product Details anti-Liver Arginase Antibody

Binding Specificity: AA 25-183

Purpose: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (ABIN7600888). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3μ/1x10⁶ cells, Human1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. Mutat. 14: 355-356, 1999. 2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic study of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989. 3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and nucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: ARG1

Background:

Synonyms: Putative Polycomb group protein ASXL1, Additional sex combs-like protein 1, ASXL1, KIAA0978

Tissue Specificity: Widely expressed at low level. Expressed in heart,brain,skeletal muscle,placenta,pancreas,spleen,prostate,mall intestine,colon,peripheral blood,leukocytes,bone marrow and fetal liver.Highly expressed in testes.

Background: ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98 % of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90 % of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50 % of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 35 kDa

Gene ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin