PDE6B antibody (AA 25-237)

Catalog No. ABIN7600892

The Mouse Monoclonal anti-PDE6B antibody (Clone 8I2D7) (ABIN7600892) specifically detects PDE6B in WB, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDE6B antibody (AA 25-237) (ABIN7600892)

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDE6B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 8I2D7

Product Details anti-PDE6B Antibody

Binding Specificity: AA 25-237

Purpose: Anti-PDE6 beta/PDE6B Antibody (monoclonal, 8I2D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDE6 beta/PDE6B Antibody (monoclonal, 8I2D7) (ABIN7600892). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDE6B

Target: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Alternative Name: PDE6B

Background:

Synonyms: Arylsulfatase A, ASA, Cerebroside-sulfatase, Arylsulfatase A component B, Arylsulfatase A component C, ARSA

Background: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 98 kDa

Gene ID: 5158

UniProt: P35913

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction