SH3BP2 antibody (AA 256-561)

Catalog No. ABIN7600936

The Rabbit Polyclonal anti-SH3BP2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect SH3BP2 in samples from Human.

Quick Overview for SH3BP2 antibody (AA 256-561) (ABIN7600936)

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3BP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SH3BP2 Antibody

Binding Specificity: AA 256-561

Purpose: Anti-3BP2/SH3BP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-3BP2/SH3BP2 Antibody Picoband® (ABIN7600936). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human 3BP2/SH3BP2 recombinant protein (Position: R256-R561).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bell, S. M., Shaw, M., Jou, Y.-S., Myers, R. M., Knowles, M. A. Identification and characterization of the human homologue of SH3BP2, an SH3 binding domain protein within a common region of deletion at 4p16.3 involved in bladder cancer. Genomics 44: 163-170, 1997. 2. Lo, B., Faiyaz-Ul-Haque, M., Kennedy, S., Aviv, R., Tsui, L.-C., Teebi, A. S. Novel mutation in the gene encoding c-Abl-binding protein SH3BP2 causes cherubism. Am. J. Med. Genet. 121A: 37-40, 2003. 3. Ueki, Y., Lin, C.-Y., Senoo, M., Ebihara, T., Agata, N., Onji, M., Saheki, Y., Kawai, T., Mukherjee, P. M., Reichenberger, E., Olsen, B. R. Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 'cherubism' mice. Cell 128: 71-83, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SH3BP2

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Alternative Name: SH3BP2

Background:

Synonyms: Ran-specific GTPase-activating protein,Ran-binding protein 1,RanBP1,RANBP1,

Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Background: SH3BP2 (SH3 domain-binding protein 2) is a protein that comes from a gene located on Chromosome 4. The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 70 kDa

Gene ID: 6452

UniProt: P78314

Pathways: TCR Signaling