ADAM28 antibody (AA 26-195)

Catalog No. ABIN7600945

The Rabbit Polyclonal anti-ADAM28 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect ADAM28 in samples from Mouse and Rat.

Quick Overview for ADAM28 antibody (AA 26-195) (ABIN7600945)

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-ADAM28 Antibody

Binding Specificity: AA 26-195

Purpose: Anti-Adam28 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Adam28 Antibody Picoband® (ABIN7600945). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat Adam28 recombinant protein (Position: K26-K195).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL,
1. Bates, E. E. M., Fridman, W. H., Mueller, C. G. F. The ADAMDEC1 (decysin) gene structure: evolution by duplication in a metalloprotease gene cluster on chromosome 8p12. Immunogenetics 54: 96-105, 2002. 2. Bridges, L. C., Tani, P. H., Hanson, K. R., Roberts, C. M., Judkins, M. B., Bowditch, R. D. The lymphocyte metalloprotease MDC-L (ADAM28) is a ligand for the integrin alpha-4/beta-1. J. Biol. Chem. 277: 3784-3792, 2002. 3. Jury, J. A., Perry, A. C. F., Hall, L. Identification, sequence analysis and expression of transcripts encoding a putative metalloproteinase, eMDC II, in human and macaque epididymis. Molec. Hum. Reprod. 5: 1127-1134, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADAM28

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Alternative Name: ADAM28

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 28,ADAM 28,3.4.24.-,Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II,eMDC II,Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L,MDC-L,ADAM28,ADAM23, MDCL,

Tissue Specificity: Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

Background: Disintegrin and metalloproteinase domain-containing protein 28 is an enzyme that in humans is encoded by the ADAM28 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. And this gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.

Molecular Weight: 87 kDa

Gene ID: 290344