TGIF1 antibody (AA 26-401)

Catalog No. ABIN7600959

This Rabbit Polyclonal antibody specifically detects TGIF1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for TGIF1 antibody (AA 26-401) (ABIN7600959)

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TGIF1 Antibody

Binding Specificity: AA 26-401

Purpose: Anti-TGIF/TGIF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TGIF/TGIF1 Antibody Picoband® (ABIN7600959). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TGIF/TGIF1 recombinant protein (Position: D26-A401).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aguilella, C., Dubourg, C., Attia-Sobol, J., Vigneron, J., Blayau, M., Pasquier, L., Lazaro, L., Odent, S., David, V. Molecular screening of the TGIF gene in holoprosencephaly: identification of two novel mutations. Hum. Genet. 112: 131-134, 2003. 2. Bertolino, E., Reimund, B., Wildt-Perinic, D., Clerc, R. G. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif. J. Biol. Chem. 270: 31178-31188, 1995. 3. Bertolino, E., Wildt, S., Richards, G., Clerc, R. G. Expression of a novel murine homeobox gene in the developing cerebellar external granular layer during its proliferation. Dev. Dyn. 205: 410-420, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TGIF1

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Alternative Name: TGIF1

Background:

Synonyms: Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hGC-1, hOLfD, OLFM4, GW112, UNQ362, PRO698

Tissue Specificity: Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Background: Homeobox protein TGIF1 is a protein that, in humans, is encoded by the TGIF1 gene. The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.

Molecular Weight: 43 kDa

Gene ID: 7050

UniProt: Q15583

Pathways: Retinoic Acid Receptor Signaling Pathway, Chromatin Binding, Tube Formation