TLR4 antibody (AA 26-827)

Catalog No. ABIN7600972

The Rabbit Polyclonal anti-TLR4 antibody has been validated for WB and ELISA. It is suitable to detect TLR4 in samples from Mouse.

Quick Overview for TLR4 antibody (AA 26-827) (ABIN7600972)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TLR4 Antibody

Binding Specificity: AA 26-827

Purpose: Anti-Tlr4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Tlr4 Antibody Picoband® (ABIN7600972). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Tlr4 recombinant protein (Position: N26-E827).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.5-1 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Aderem, A., Ulevitch, R. J. Toll-like receptors in the induction of the innate immune response. Nature 406: 782-787, 2000. 2. Andonegui, G., Bonder, C. S., Green, F., Mullaly, S. C., Zbytnuik, L., Raharjo, E., Kubes, P. Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs. J. Clin. Invest. 111: 1011-1020, 2003. Note: Erratum: J. Clin. Invest. 112: 1264 only, 2003. 3. Andonegui, G., Zhou, H., Bullard, D., Kelly, M. M., Mullaly, S. C., McDonald, B., Long, E. M., Robbins, S. M., Kubes, P. Mice that exclusively express TLR4 on endothelial cells can efficiently clear a lethal systemic gram-negative bacterial infection. J. Clin. Invest. 119: 1921-1930, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: Tlr4

Background:

Synonyms: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1

Tissue Specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Background: Toll-like receptor 4 is a protein that in humans is encoded by the TLR4 gene. This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.

Molecular Weight: 96 kDa

Gene ID: 21898

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins