POLA2 antibody (AA 267-579)

Catalog No. ABIN7600985

This anti-POLA2 antibody is a Rabbit Polyclonal antibody detecting POLA2 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for POLA2 antibody (AA 267-579) (ABIN7600985)

Target: POLA2 (Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POLA2 Antibody

Binding Specificity: AA 267-579

Purpose: Anti-POLA2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POLA2 Antibody Picoband® (ABIN7600985). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLA2 recombinant protein (Position: N267-P579).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ste Fa N, M. , L Agnès, Thomas, B. , Philippe, D. , Bertrand, G. , & Arnaud, M. , et al. (2009). Genetic polymorphisms in 85 dna repair genes and bladder cancer risk. Carcinogenesis, 30(5), 763-768. 2. Beausoleil, S., Villén, J., Gerber, S. et al. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-1292 (2006). 3. Stelzl, U. , Worm, U. , Lalowski, M. , Haenig, C. , Brembeck, F. H. , & Goehler, H. , et al. (2005). A human protein-protein interaction network: a resource for annotating the proteome. Cell, 122(6), 957-968.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLA2

Target: POLA2 (Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2))

Alternative Name: POLA2

Background:

Synonyms: Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: DNA polymerase alpha subunit 2 is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).

Molecular Weight: 70 kDa

Gene ID: 23649

UniProt: Q14181

Pathways: Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Protein targeting to Nucleus, Synthesis of DNA, SARS-CoV-2 Protein Interactome