HMGCR antibody (AA 268-842)

Catalog No. ABIN7600989

This Rabbit Polyclonal antibody specifically detects HMGCR in WB, IHC, ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for HMGCR antibody (AA 268-842) (ABIN7600989)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-HMGCR Antibody

Binding Specificity: AA 268-842

Purpose: Anti-HMGCR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HMGCR Antibody Picoband® (ABIN7600989). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HMGCR recombinant protein (Position: H268-V842).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aulchenko, Y. S., Ripatti, S., Lindqvist, I., Boomsma, D., Heid, I. M., Pramstaller, P. P., Penninx, B. W. J. H., Janssens, A. C. J. W., Wilson, W. F., Spector, T., Martin, N. G., Pedersen, N. L. {and 45 others}: Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts. Nature Genet. 41: 47-55, 2009. 2. Chasman, D. I., Posada, D., Subrahmanyan, L., Cook, N. R., Stanton, V. P., Jr., Ridker, P. M. Pharmacogenetic study of statin therapy and cholesterol reduction. JAMA 291: 2821-2827, 2004. 3. Goldstein, J. L., Brown, M. S. Regulation of the mevalonate pathway. Nature 343: 425-430, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Alternative Name: HMGCR

Background:

Synonyms: Major vault protein, MVP, Lung resistance-related protein, MVP, LRP

Tissue Specificity: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

Background: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 97 kDa

Gene ID: 3156

UniProt: P04035

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha