HMGCR antibody (full length)

Catalog No. ABIN2443869

The Rabbit Polyclonal anti-HMGCR antibody (ABIN2443869) specifically detects HMGCR in WB and IF. The antibody is reactive with Rat samples.

Quick Overview for HMGCR antibody (full length) (ABIN2443869)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HMGCR Antibody

Binding Specificity: full length

Purpose: HMG Co A reductase Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the HMG CoA reductase

Immunogen: Full length protein

Application Details

Application Notes: WB 1:500,IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:500, IF 1:50-1:100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Alternative Name: HMG Co A reductase

Background:

3-hydroxy-3-methyl-glutaryl-CoA reductase, HMGCR

HMG-CoA reductase (also known as 3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGCR) is a transmembrane glycoprotein involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis, it is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum. HMG-CoA reductase is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. HMG-CoA is inhibited by statin, a class of cholesterol-lowering drugs which lower plasma cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor.

UniProt: P51639

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha