ACCN1 antibody (AA 27-512)

Catalog No. ABIN7601023

The Rabbit Polyclonal anti-ACCN1 antibody has been validated for WB, ELISA, FACS, IF and ICC. It is suitable to detect ACCN1 in samples from Human, Rat and Mouse.

Quick Overview for ACCN1 antibody (AA 27-512) (ABIN7601023)

Target: ACCN1 (Amiloride-Sensitive Cation Channel 1, Neuronal (ACCN1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACCN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ACCN1 Antibody

Binding Specificity: AA 27-512

Purpose: Anti-ACCN1/ASIC2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACCN1/ASIC2 Antibody Picoband® (ABIN7601023). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACCN1/ASIC2 recombinant protein (Position: H27-C512).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Garcia-Anoveros, J., Derfler, B., Neville-Golden, J., Hyman, B. T., Corey, D. P. BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels. Proc. Nat. Acad. Sci. 94: 1459-1464, 1997. 2. Jahr, H., van Driel, M., van Osch, G. J. V. M., Weinans, H., van Leeuwen, J. P. T. M. Identification of acid-sensing ion channels in bone. Biochem. Biophys. Res. Commun. 337: 349-354, 2005. 3. Lu, Y., Ma, X., Sabharwal, R., Snitsarev, V., Morgan, D., Rahmouni, K., Drummond, H. A., Whiteis, C. A., Costa, V., Price, M., Benson, C., Welsh, M. J., Chapleau, M. W., Abboud, F. M. The ion channel ASIC2 is required for baroreceptor and autonomic control of the circulation. Neuron 64: 885-897, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACCN1

Target: ACCN1 (Amiloride-Sensitive Cation Channel 1, Neuronal (ACCN1))

Alternative Name: ASIC2

Background:

Synonyms: Acid-sensing ion channel 2, ASIC2, Amiloride-sensitive brain sodium channel, Amiloride-sensitive cation channel 1, neuronal, Amiloride-sensitive cation channel neuronal 1, Brain sodium channel 1, BNC1, BNaC1, Mammalian degenerin homolog, MDEG, ASIC2, ACCN, ACCN1, BNAC1, MDEG

Tissue Specificity: Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.

Background: Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2,?is a?protein?that in?humans?is encoded by the?ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.?

Molecular Weight: 58 kDa

Gene ID: 40

UniProt: Q16515

Pathways: Sensory Perception of Sound