CD31 antibody (AA 28-382)

Catalog No. ABIN7601076

This Mouse Monoclonal antibody specifically detects CD31 in WB, IHC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD31 antibody (AA 28-382) (ABIN7601076)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2D4

Product Details anti-CD31 Antibody

Binding Specificity: AA 28-382

Purpose: Anti-CD31/PECAM1 Antibody (monoclonal, 2D4)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human CD31 shares 65%,68% amino acid (aa) sequences identity with mouse,rat CD31,respectively.

Characteristics: Anti-CD31/PECAM1 Antibody.(monoclonal, 2D4)-4. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background:

Background: CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Gene Full Name: platelet and endothelial cell adhesion molecule 1

Molecular Weight: 130 kDa

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization