ADGRE5 (AA 287-816) antibody

Catalog No. ABIN7601103

This Rabbit Polyclonal antibody specifically detects in WB, ELISA, FACS and IHC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for ADGRE5 (AA 287-816) antibody (ABIN7601103)

Target: ADGRE5

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 287-816

Purpose: Anti-CD97/Adgre5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD97/Adgre5 Antibody Picoband® (ABIN7601103). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD97/Adgre5 recombinant protein (Position: Q287-S816).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Hamann, J., Eichler, W., Hamann, D., Kerstens, H. M. J., Poddighe, P. J., Hoovers, J. M. N., Hartmann, E., Strauss, M., van Lier, R. A. W. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain. J. Immun. 155: 1942-1950, 1995. 2. Hamann, J., Hartmann, E., van Lier, R. A. W. Structure of the human CD97 gene: exon shuffling has generated a new type of seven-span transmembrane molecule related to the secretin receptor superfamily. Genomics 32: 144-147, 1996. 3. Hamann, J., Vogel, B., van Schijndel, G. M., van Lier, R. A. The seven-span transmembrane receptor CD97 has a cellular ligand (CD55, DAF). J. Exp. Med. 184: 1185-1189, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: ADGRE5

Background:

Synonyms: C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6, CCL6 (22-95), CCL6 (23-95), Ccl6, C10, Scya6

Tissue Specificity: Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

Background: Cluster of differentiation 97 is a protein also known as BL-Ac[F2] encoded by the ADGRE5 gene. This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19.

Molecular Weight: 92 kDa

Gene ID: 26364

UniProt: Q9Z0M6