MED29 antibody (AA 29-184)

Catalog No. ABIN7601107

The Rabbit Polyclonal anti-MED29 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect MED29 in samples from Human.

Quick Overview for MED29 antibody (AA 29-184) (ABIN7601107)

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MED29 Antibody

Binding Specificity: AA 29-184

Purpose: Anti-MED29 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED29 Antibody Picoband® (ABIN7601107). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED29 recombinant protein (Position: Q29-K184).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Parmely, T. J., Sorokina, I., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian homolog of Drosophila melanogaster transcriptional coactivator Intersex is a subunit of the mammalian Mediator complex. J. Biol. Chem. 278: 49671-49674, 2003. 2. Wang, Y., Li, Y., Zeng, W., Zhu, C., Xiao, J., Yuan, W., Wang, Y., Cai, Z., Zhou, J., Liu, M., Wu, X. IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor. Biochem. Biophys. Res. Commun. 325: 1330-1338, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MED29

Target: MED29 (Mediator Complex Subunit 29 (MED29))

Alternative Name: MED29

Background:

Synonyms: Neutrophil defensin 1,Defensin, alpha 1,HNP-1,HP-1,HP1,HP 1-56,Neutrophil defensin 2,HNP-2,HP-2,HP2,DEFA1,DEF1, DEFA2, MRS,DEFA1B,

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Mediator of RNA polymerase II transcription subunit 29 (Med29) is a transcription suppressor that in humans is encoded by the MED29 gene. MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II and general initiation factors.

Molecular Weight: 21 kDa

Gene ID: 55588

Pathways: Regulation of Lipid Metabolism by PPARalpha