GRB7 antibody (AA 29-256)

Catalog No. ABIN7601118

This Rabbit Polyclonal antibody specifically detects GRB7 in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for GRB7 antibody (AA 29-256) (ABIN7601118)

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GRB7 Antibody

Binding Specificity: AA 29-256

Purpose: Anti-GRB7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GRB7 Antibody Picoband® (ABIN7601118). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRB7 recombinant protein (Position: R29-S256).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Tanaka, S., Mori, M., Akiyoshi, T., Tanaka, Y., Mafune, K., Wands, J. R., Sugimachi, K.A novel variant of human Grb7 is associated with invasive esophageal carcinoma.J. Clin. Invest. 102: 821-827, 1998. 2. Vayssiere, B., Zalcman, G., Mahe, Y., Mirey, G., Ligensa, T., Weidner, K. M., Chardin, P., Camonis, J.Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family.FEBS Lett. 467: 91-96, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRB7

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Alternative Name: GRB7

Background:

Synonyms: Natural killer cell receptor 2B4, Cd244, LOC685808

Tissue Specificity: Expressed in natural killer (NK) cells, T cells and dendritic cells.

Background: GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 though to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

Molecular Weight: 60 kDa

Gene ID: 2886

UniProt: Q14451

Pathways: EGFR Signaling Pathway, Ribonucleoprotein Complex Subunit Organization