Integrin beta 3 antibody (AA 29-523)

Catalog No. ABIN7601139

This Rabbit Polyclonal antibody specifically detects Integrin beta 3 in WB, IHC, FACS and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for Integrin beta 3 antibody (AA 29-523) (ABIN7601139)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Product Details anti-Integrin beta 3 Antibody

Binding Specificity: AA 29-523

Purpose: Anti-Integrin beta 3/ITGB3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Integrin beta 3/ITGB3 Antibody Picoband® (ABIN7601139). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin beta 3/ITGB3 recombinant protein (Position: N29-Q523).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Andre, P., Srinivasa Prasad, K. S., Denis, C. V., He, M., Papalia, J. M., Hynes, R. O., Phillips, D. R., Wagner, D. D. CD40L stabilizes arterial thrombi by a beta(3) integrin-dependent mechanism. Nature Med. 8: 247-252, 2002. 2. Anderson, L. A., Friedman, L., Osborne-Lawrence, S., Lynch, E., Weissenbach, J., Bowcock, A., King, M.-C. High-absorbance genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics 17: 618-623, 1993. 3. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988). "Chromosomal localization of the genes for the vitronectin and fibronectin receptors alpha subunits and for platelet glycoproteins IIb and IIIa".

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3

Background:

Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF

Background: ITGB3 (INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. It is a cluster of differentiation found on thrombocytes. This gene is mapped to 17q21.32. And the GP3A gene has 14 exons. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. Additionally, the ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Molecular Weight: 100 kDa

Gene ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex