RCC2 antibody (AA 293-522)

Catalog No. ABIN7601154

This Rabbit Polyclonal antibody specifically detects RCC2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for RCC2 antibody (AA 293-522) (ABIN7601154)

Target: RCC2 (Regulator of Chromosome Condensation 2 (RCC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RCC2 Antibody

Binding Specificity: AA 293-522

Purpose: Anti-RCC2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RCC2 Antibody Picoband® (ABIN7601154). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RCC2 recombinant protein (Position: K293-L522).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. P. R., Boulet, A., Goud, B., Kleman, J.-P., Margolis, R. L. The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression. Dev. Cell 5: 295-307, 2003. 2. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 143-150, 2000. 3. Rosasco-Nitcher, S. E., Lan, W., Khorasanizadeh, S., Stukenberg, P. T. Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation. Science 319: 469-472, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RCC2

Target: RCC2 (Regulator of Chromosome Condensation 2 (RCC2))

Alternative Name: RCC2

Background:

Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB

Tissue Specificity: Brain, neural-specific.

Background: Protein RCC2 also known as telophase disk protein of 60 kDa (TD-60) or RCC1-like protein TD-60 is a protein that in humans is encoded by the RCC2 gene. The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer.

Molecular Weight: 56 kDa

Gene ID: 55920

UniProt: Q9P258