NCALD antibody (AA 3-193)

Catalog No. ABIN7601173

The Rabbit Polyclonal anti-NCALD antibody has been validated for WB and ELISA. It is suitable to detect NCALD in samples from Human, Mouse and Rat.

Quick Overview for NCALD antibody (AA 3-193) (ABIN7601173)

Target: NCALD (Neurocalcin delta (NCALD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCALD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NCALD Antibody

Binding Specificity: AA 3-193

Purpose: Anti-NCALD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NCALD Antibody Picoband® (ABIN7601173). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NCALD recombinant protein (Position: K3-F193).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Riessland, M., Kaczmarek, A., Schneider, S., Swoboda, K. J., Lohr, H., Bradler, C., Grysko, V., Dimitriadi, M., Hosseinibarkooie, S., Torres-Benito, L., Peters, M., and 17 others. Neurocalcin delta suppression protects against spinal muscular atrophy in humans and across species by restoring impaired endocytosis. Am. J. Hum. Genet. 100: 297-315, 2017. 2. Wang, W., Zhou, Z., Zhao, W., Huang, Y., Tang, R., Ying, K., Xie, Y., Mao, Y. Molecular cloning, mapping and characterization of the human neurocalcin delta gene (NCALD). Biochim. Biophys. Acta 1518: 162-167, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCALD

Target: NCALD (Neurocalcin delta (NCALD))

Alternative Name: NCALD

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels, however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein, additional variants may exist but their biological validity has not been determined.

Molecular Weight: 22 kDa

Gene ID: 83988

UniProt: P61601