Caspase 9 antibody (AA 3-266)

Catalog No. ABIN7601182

The Rabbit Polyclonal anti-Caspase 9 antibody is suitable to detect Caspase 9 in samples from Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for Caspase 9 antibody (AA 3-266) (ABIN7601182)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Caspase 9 Antibody

Binding Specificity: AA 3-266

Purpose: Anti-Caspase-9 p35/Casp9 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Caspase-9 p35/Casp9 Antibody (ABIN7601182). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Caspase-9 p35/Casp9 recombinant protein (Position: E3-D266).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Casp9

Background:

Synonyms: Interleukin-13,IL-13,IL13,NC30,

Tissue Specificity: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Background: Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Molecular Weight: 37/46 kDa

Gene ID: 12371

UniProt: Q8C3Q9

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity