CRTC1 antibody (AA 30-568)

Catalog No. ABIN7601228

This anti-CRTC1 antibody is a Rabbit Polyclonal antibody detecting CRTC1 in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CRTC1 antibody (AA 30-568) (ABIN7601228)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-CRTC1 Antibody

Binding Specificity: AA 30-568

Purpose: Anti-TORC1/CRTC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TORC1/CRTC1 Antibody Picoband® (ABIN7601228). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TORC1/CRTC1 recombinant protein (Position: E30-E568).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kovacs, K. A. Steullet, P., Steinmann, M., Do, K. Q., Magistretti, P. J., Halfon, O., Cardinaux, J.-R. TORC1 is a calcium- and cAMP-sensitive coincidence detector involved in hippocampal long-term synaptic plasticity. Proc. Nat. Acad. Sci. 104: 4700-4705, 2007. 2. Mair, W., Morantte, I., Rodrigues, A. P. C., Manning, G., Montminy, M., Shaw, R. J., Dillin, A. Lifespan extension induced by AMPK and calcineurin is mediated by CRTC-1 and CREB. Nature 470: 404-408, 2011. 3. Tonon, G., Modi, S., Wu, L., Kubo, A., Coxon, A. B., Komiya, T., O'Neil, K., Stover, K., El-Naggar, A., Griffin, J. D., Kirsch, I. R., Kaye, F. J. t(11,19)(q21,p13) translocation in mucoepidermoid carcinoma creates a novel fusion product that disrupts a Notch signaling pathway. Nature Genet. 33: 208-213, 2003. Note: Erratum: Nature Genet. 33: 430 only, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1

Background:

Synonyms: CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1, CRTC1, KIAA0616

Tissue Specificity: Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.

Background: CRTC1 (CREB-Regulated Transcription Coactivator 1), also known as MECT1, TORC1 or KIAA0616, is a protein that in humans is encoded by the CRTC1 gene. By sequence analysis, Tonon et al. (2003) mapped the CRTC1 gene to chromosome 19p13. Kovacs et al. (2007) found that Torc1 was expressed in adult mouse brain and cultured neurons, and that it translocated to the nucleus upon concomitant activation of calcium and cAMP signaling pathways. Mair et al. (2011) demonstrated that CRTC1 is a AMPK target, and interacts with the CREB homolog-1 (CRH1) transcription factor in vivo. The prolongevity effects of activating AMPK or deactivating calcineurin decrease CRTC1 and CRH1 activity and induce transcriptional responses similar to those of CRH1-null worms.

Molecular Weight: 78 kDa

Gene ID: 23373