CD16 antibody (AA 31-215)

Catalog No. ABIN7601253

This Rabbit Polyclonal antibody specifically detects CD16 in FACS, WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for CD16 antibody (AA 31-215) (ABIN7601253)

Target: CD16

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-CD16 Antibody

Binding Specificity: AA 31-215

Purpose: Anti-CD16/Fcgr3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD16/Fcgr3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD16/Fcgr3 recombinant protein (Position: A31-T215).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: Fcgr3

Background:

Background: Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Gene Full Name: Fc receptor, IgG, low affinity III

Molecular Weight: 30 kDa

Gene ID: 14131

UniProt: P08508

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process