MYBL2 antibody (AA 31-695)

Catalog No. ABIN7601277

This Rabbit Polyclonal antibody specifically detects MYBL2 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for MYBL2 antibody (AA 31-695) (ABIN7601277)

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYBL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MYBL2 Antibody

Binding Specificity: AA 31-695

Purpose: Anti-B MyB/MYBL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-B MyB/MYBL2 Antibody Picoband® (ABIN7601277). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human B MyB/MYBL2 recombinant protein (Position: K31-R695).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barletta, C., Druck, T., LaForgia, S., Calabretta, B., Drabkin, H., Patterson, D., Croce, C. M., Huebner, K. Chromosome locations of the MYB related genes, AMYB and BMYB. Cancer Res. 51: 3821-3824, 1991. 2. Noben-Trauth, K., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Sonoda, G., Testa, J. R., Klempnauer, K.-H. Mybl2 (Bmyb) maps to mouse chromosome 2 and human chromosome 20q13.1. Genomics 35: 610-612, 1996. 3. Shepard, J. L., Amatruda, J. F., Stern, H. M., Subramanian, A., Finkelstein, D., Ziai, J., Finley, K. R., Pfaff, K. L., Hersey, C., Zhou, Y., Barut, B., Freedman, M., and 9 others. A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility. Proc. Nat. Acad. Sci. 102: 13194-13199, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MYBL2

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Alternative Name: MYBL2

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 15, ADAM 15, Metalloprotease RGD disintegrin protein, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, MDC-15, Metargidin, ADAM15, MDC15

Tissue Specificity: Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.

Background: MYBL2 (V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al. (1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al. (1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Molecular Weight: 100 kDa

Gene ID: 4605

UniProt: P10244

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Chromatin Binding